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large construct kit dna sequencing  (Qiagen)


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    Structured Review

    Qiagen large construct kit dna sequencing
    Large Construct Kit Dna Sequencing, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1465 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/large+construct+kit+dna+sequencing/QIAGEN+Large-Construct+Kit/pm41813157-161-7-6
    Average 97 stars, based on 1465 article reviews
    large construct kit dna sequencing - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    BAC Assay:

    Article Title: Comparative sequence analysis of the mouse pseudoautosomal region from three inbred strains reveals it to be the most rapidly evolving 'chromosome'.
    Article Snippet: .. The BAC DNA was purified using QIAGEN Large-Construct Kit DNA sequencing by primer walking initially started from both ends of the BAC clone and from every exon of Mid1, Sts, and Asmt genes. .. Since the mouse PAR is mostly GCrich, we always used a 1:1 mixture of dGTP BigDye Terminator v3.0 (Applied Biosystems) and BigDye Terminator v3.1 Cycle Sequencing kits supplemented with dimethyl sulfoxide (final 10%(v/v)).

    Article Title: Comparative sequence analysis of the mouse pseudoautosomal region from three inbred strains reveals it to be the most rapidly evolving ‘chromosome’
    Article Snippet: .. The BAC DNA was purified using QIAGEN Large-Construct Kit DNA sequencing by primer walking initially started from both ends of the BAC clone and from every exon of Mid1 , Sts , and Asmt genes. .. Since the mouse PAR is mostly GC-rich, we always used a 1:1 mixture of dGTP BigDye Terminator v3.0 (Applied Biosystems) and BigDye Terminator v3.1 Cycle Sequencing kits supplemented with dimethyl sulfoxide (final 10%(v/v)).

    Purification:

    Article Title: Comparative sequence analysis of the mouse pseudoautosomal region from three inbred strains reveals it to be the most rapidly evolving 'chromosome'.
    Article Snippet: .. The BAC DNA was purified using QIAGEN Large-Construct Kit DNA sequencing by primer walking initially started from both ends of the BAC clone and from every exon of Mid1, Sts, and Asmt genes. .. Since the mouse PAR is mostly GCrich, we always used a 1:1 mixture of dGTP BigDye Terminator v3.0 (Applied Biosystems) and BigDye Terminator v3.1 Cycle Sequencing kits supplemented with dimethyl sulfoxide (final 10%(v/v)).

    Article Title: Comparative sequence analysis of the mouse pseudoautosomal region from three inbred strains reveals it to be the most rapidly evolving ‘chromosome’
    Article Snippet: .. The BAC DNA was purified using QIAGEN Large-Construct Kit DNA sequencing by primer walking initially started from both ends of the BAC clone and from every exon of Mid1 , Sts , and Asmt genes. .. Since the mouse PAR is mostly GC-rich, we always used a 1:1 mixture of dGTP BigDye Terminator v3.0 (Applied Biosystems) and BigDye Terminator v3.1 Cycle Sequencing kits supplemented with dimethyl sulfoxide (final 10%(v/v)).

    DNA Sequencing:

    Article Title: Comparative sequence analysis of the mouse pseudoautosomal region from three inbred strains reveals it to be the most rapidly evolving 'chromosome'.
    Article Snippet: .. The BAC DNA was purified using QIAGEN Large-Construct Kit DNA sequencing by primer walking initially started from both ends of the BAC clone and from every exon of Mid1, Sts, and Asmt genes. .. Since the mouse PAR is mostly GCrich, we always used a 1:1 mixture of dGTP BigDye Terminator v3.0 (Applied Biosystems) and BigDye Terminator v3.1 Cycle Sequencing kits supplemented with dimethyl sulfoxide (final 10%(v/v)).

    Article Title: Comparative sequence analysis of the mouse pseudoautosomal region from three inbred strains reveals it to be the most rapidly evolving ‘chromosome’
    Article Snippet: .. The BAC DNA was purified using QIAGEN Large-Construct Kit DNA sequencing by primer walking initially started from both ends of the BAC clone and from every exon of Mid1 , Sts , and Asmt genes. .. Since the mouse PAR is mostly GC-rich, we always used a 1:1 mixture of dGTP BigDye Terminator v3.0 (Applied Biosystems) and BigDye Terminator v3.1 Cycle Sequencing kits supplemented with dimethyl sulfoxide (final 10%(v/v)).

    Chromosome Walking:

    Article Title: Comparative sequence analysis of the mouse pseudoautosomal region from three inbred strains reveals it to be the most rapidly evolving 'chromosome'.
    Article Snippet: .. The BAC DNA was purified using QIAGEN Large-Construct Kit DNA sequencing by primer walking initially started from both ends of the BAC clone and from every exon of Mid1, Sts, and Asmt genes. .. Since the mouse PAR is mostly GCrich, we always used a 1:1 mixture of dGTP BigDye Terminator v3.0 (Applied Biosystems) and BigDye Terminator v3.1 Cycle Sequencing kits supplemented with dimethyl sulfoxide (final 10%(v/v)).

    Article Title: Comparative sequence analysis of the mouse pseudoautosomal region from three inbred strains reveals it to be the most rapidly evolving ‘chromosome’
    Article Snippet: .. The BAC DNA was purified using QIAGEN Large-Construct Kit DNA sequencing by primer walking initially started from both ends of the BAC clone and from every exon of Mid1 , Sts , and Asmt genes. .. Since the mouse PAR is mostly GC-rich, we always used a 1:1 mixture of dGTP BigDye Terminator v3.0 (Applied Biosystems) and BigDye Terminator v3.1 Cycle Sequencing kits supplemented with dimethyl sulfoxide (final 10%(v/v)).



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